Anna Sannino
Anna Sannino
Anna
Sannino
...
+39 081-76206 58
Technical staff

Activity

The research activity of Dr. Sannino deals with the evaluation of cytotoxic and genotoxic effects induced in mammalian cell cultures following exposures to low and high frequency electromagnetic fields (EMF). In particular, the research activity consists in the evaluation of the effects induced in human lymphocytes (proliferating or quiescent) and in mammalian cell lines after exposures to electromagnetic fields at mobile phone frequencies (900 MHz and 1950 MHz), with different signal characteristics (GSM, UMTS) and Specific Absorption Rate (SAR). The experimental activity is carried out by employing different exposure systems (TEM cell, WPC, waveguide), mode and duration (continuous or intermittent exposures). Moreover, Dr Sannino took part in the study about the evaluation of the genotoxic effects of electromagnetic fields in the THz frequency range (130 GHz), while the effects of low-frequency EM fields have been explored by studying the induction of genotoxic effects in human fibroblast non-trasformed cell line following 24 h exposures to a 50 Hz frequency, 1 mT amplitude, magnetic field.Currently, the research activity of Dr Sannino is mainly focused on: Evaluation of the adaptive response induced in human lymphocytes following exposures to RF fields (900 – 1950 MHz), given as adaptive doses, and subsequently treated with high doses of chemical and physical mutagens. Cytogenetic assays (micronucleus with cytokinesis block and comet assay) are employed for the study and characterization of the adaptive response;Evaluation of the cytotoxicity in rat adrenal phaeochromocytoma (PC12) cell line following exposures to 1950 MHz, UMTS signal, RF fields. To this aim, different assays are employed to investigate cell viability by means of microscopic, spectrofluorimetric and fluorimetric techniques (Trypan Blue, Resazurin, Neutral Red).  Evaluation of the oxidative stress and apoptosis induction in different mammalian cell lines exposed to RF fields, by applying fluorimetric and cytometric techniques;  evaluation of the cytotoxicity induced by multiwalled carbon-nanotubes (buckypaper) and magnetic nanoparticles in mammalian cell cultures by applying fluorimetric techniques and confocal microscopy;  evaluation of the cellular and sub-cellular effects induced by high voltage, nanosecond electric pulses in Jurkat cells by means of molecular cytogenetic assays, cytofluorimetry and confocal microscopy.

Curriculum

Anna Sannino was born in Castellammare di Stabia (Naples) in 1975. In 2002, she graduated in Biology at the University of Naples, Federico II with a thesis entitled: “Evaluation of the genotoxic effects in human lymphocytes exposed to electromagnetic fields at mobile phone frequency (900 MHz)”.  She attended the “Fluorimetry and biological applications” (by Perkin Elmer) and “The Comet assay technique: automated image analysis and data interpretation” (by Delta Sistemi, Rome) courses, in 2002 and 2003, respectively. Dr Sannino attended the 1st and the 2nd courses of the International School of Bioelectromagnetics “Alessandro Chiabrera” held in Erice (Italy), in 2004 and 2005, respectively, and the training course “Electromagnetic fields: risk and protection” held in Naples, Federico II, in 2006. In 2010, she attended the “11th Practical Intensive Workshop on 3D Canfocal and Multiphoton Microscopy” and the “6th Confocal Microscopy course: theoretical and practical basis”. In 2013, she recived PhD in “Biochemistry and pathology of drug action”  at the University of Salerno. Since 2002, she has been working at the Bioelectromagnetics Laboratory of the IREA-CNR, and since 2017 she has been a techical staff dealing with the evaluation of the biological effects of low and high-frequency electromagnetic fields.

Technical staf

Scientific production

ISI

 

A. Sannino, O. Zeni, S. Romeo, R. Massa, MR. Scarfi. "Adverse and beneficial effects in Chinese hamster lung fibroblast cells following radiofrequency exposure". Bioelectromagnetics. 2017. doi: 10.1002/bem.22034. [Epub ahead of print]

 

S. Romeo, A. Sannino, M.R. Scarfì, R. Massa, R. d’Angelo, O. Zeni, “Lack of effects on key cellular parameters of MRC-5 human lung fibroblasts exposed to 370 mT static magnetic field”, Sci Rep. 2016 14;6:19398. doi: 10.1038/srep19398.

  

P. Lamberti, S. Romeo, A. Sannino, L. Zeni, O. Zeni, “The Role of Pulse Repetition Rate in nsPEF-induced Electroporation: A Biological and Numerical Investigation”. IEEE Trans Biomed Eng. 2015. [Epub ahead of print]

 

A. Sannino, O. Zeni , S. Romeo , R. Massa , G. Gialanella , G. Grossi , L. Manti , Vijayalaxmi, MR. Scarfì. “Adaptive response in human blood lymphocytes exposed to non-ionizing radiofrequency fields: resistance to ionizing radiation-induced damage”. J Radiat Res. 2013.

 

P. Lamberti, V. Tucci, S. Romeo, A. Sannino, M. R. Scarfì, O. Zeni. “nsPEF-induced effects on cell membranes: the use of an electrophysical model to optimize the experimental design”, IEEE Trans. on Dielectrics and Electrical Insulation, Vol. 20, Issue 4, pp. 1231-1238, 2013.  

 

O. Zeni, A. Sannino, S. Romeo, R. Massa, M. Sarti, A.B. Reddy, T.J. Prihoda, Vijayalaxmi and M.R. Scarfì, “Induction of Adaptive Response in Human Blood Lymphocytes Exposed to Radiofrequency Fields: Influence of UMTS Signal and Specific Absorption Rate”, Mutation Research – Genetic Toxicology and Environmental Mutagenesis, 747, 29-35, 2012.


O. Zeni, A. Sannino, M. Sarti, S. Romeo, R. Massa, M.R. Scarfì, “Radiofrequency radiation at 1950 MHz, UMTS signal, does not affect some cellular endpoints relevant for carcinogenesis in neural-like PC12 cells”, Bioelectromagnetics, 2012 Sep;33(6):497-507.

 

S. Romeo, L. Zeni, M. Sarti, A. Sannino, M.R. Scarfì, P.T. Vernier, O. Zeni, “DNA
electrophoretic migration patterns change after exposure of Jurkat cells to a single intense nanosecond electric pulse”, PLoSONE, 6, 12, 2011.:e28419. doi:10.1371/journal.pone.

 

A. Sannino, O. Zeni, M. Sarti, S. Romeo, S.B. Reddy, A. Belisario, T.J. Prihoda, Vijayalaxmi, M.R. Scarfì, “Characterization of Radiofrequency-induced Adaptive Response in human peripheral blood lymphocytes: cell cycle effects”, International Journal of Radiation Biology, 87, 7, 1–8, 2011.

 

A. Sannino, M. Sarti, S.B. Reddy, T.J. Prihoda, Vijayalaxmi, M.R. Scarfì, “Induction of Adaptive Response in Human Blood Lymphocytes Exposed to Radiofrequency Radiation”, Radiation Research, 171, 735–742, 2009.

 

A. Sannino, G. Di Costanzo, F. Brescia, M. Sarti, O. Zeni, J. Juutilainenb, M. R. Scarfı`, “Human Fibroblasts and 900 MHz Radiofrequency Radiation: Evaluation of DNA Damage after Exposure and Co-exposure to 3-Chloro-4-(dichloromethyl)-5-Hydroxy-2(5h)-furanone (MX)”, Radiation Research 171, 743–751, 2009.

 

F. Brescia, M. Sarti, R. Massa, M. Calabrese, A. Sannino, M.R. Scarfì, “Reactive oxygen species formation is not enhanced by exposure to UMTS 1950 MHz radiation and co-exposure to ferrous ions in Jurkat cells”, Bioelectromagnetics, 30, 525-535, 2009.

 

R. Palumbo, F. Brescia, D. Capasso, A. Sannino, M. Sarti, M. Capri, E. Grassilli, M.R. Scarfì, “Exposure to 900 MHz radiofrequency radiation induces caspase-3 activation in proliferating human lymphocytes”, Radiation Research, 170, 327-334, 2008.

 

O. Zeni, G.P. Gallerano, A. Perrotta, M. Romanò, A. Sannino, M. Sarti, M. D’Arienzo, A. Doria, E. Giovenale, A. Lai, G. Messina, M.R. Scarfì, “Cytogenetic observations in human peripheral blood leukocytes following in vitro exposure to THz radiation: a pilot study”, Health Physics, 92, 4, 349-357, 2007.

 

A. Sannino, M.L. Calabrese, G. d’Ambrosio, R. Massa, G. Petraglia, P. Mita, M. Sarti, M.R. Scarfì, “Evaluation of cytotoxic and genotoxic effects in human peripheral blood leukocytes following exposure to 1950 MHz modulated signal”, IEEE Transactions on Plasma Science, 34, 1441-1448, 2006.

 

M.R. Scarfì, A.M. Fresegna, P. Villani, R. Pinto, C. Marino, M. Sarti, A. Sannino, P. Altavista, G.A. Lovisolo, “Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes”, Radiation Research, 165, 655-663, 2006.

 

M.R. Scarfì, A. Sannino, A. Perrotta, M. Sarti, P. Mesirca, F. Bersani, “Evaluation of Genotoxic Effects on Human Fibroblasts Following Intermittent Exposures to 50 Hz Electromagnetic Fields: a Confirmation Study”, Radiation Research,164, 3, 270-276, 2005.

 

O. Zeni, A. Schiavoni, A. Sannino, A. Antolini, D. Forigo, F. Bersani, M.R. Scarfì, “Lack of genotoxic effects (micronucleus induction) in human lymphocytes exposed in vitro to 900 MHz electromagnetic fields”, Radiation Research, 160, 152-158, 2003.

 

Proceedings  

P. Lamberti, S. Romeo, A. Sannino, M.R. Scarfì, V. Tucci, O. Zeni, Pore dynamics induced by nsPEFs: a comparison between experimental and theoretical results, Proceedings of the 2012 IEEE International Power Modulator and High Voltage Conference, June 3-7, 2012, San Diego, CA.

O. Zeni, A. Sannino, M. Sarti, R. Massa, M.R. Scarfì, “Cytotoxicity Investigation on Neural Like PC12 Cells Exposed to UMTS 1950 MHz Radiofrequency Radiation”, in “Proceedings of the 6° International Workshop on Biological effects of Electromagnetic fields, Bodrum, Turchia, 10-14 October 2010.

 

O. Zeni, S. Romeo, M.R. Scarfì, M. Sarti, A. Sannino, P.T. Vernier, L. Zeni, Intracellular Effects of Nanosecond Pulsed Electric Fields on Jurkat Cells, Proceedings of the XVIII RINEM 2010, September 6-10, Benevento

 

A. Sannino, O. Zeni, M. Sarti, Vijayalaxmi, S. Romeo, M.R. Scarfì, “Radiofrequency radiation is capable of inducing Adaptive Response in human blood lymphocytes”, in “Proceedings of the European Conference on Antennas and Propagation 2010”, Barcelona, Spain, 12-16 April 2010.

 

 

 O. Zeni, A. Sannino, S. Romeo, M.R. Scarfì, S. Bellucci, F. Micciulla, I. Sacco, L. Coderoni, “Cytotoxicity of Buckypaper in Human Lymphocytes”, in Proceedings of the xxx Italian Conference Sensors and Microsystems, 2010.

 

R. Palumbo, F. Brescia, D. Capasso, A. Sannino, F. Bersani, M. Sarti, M.R. Scarfì, “Exposure to 900MHz radiofrequency radiation induces caspase-3 activation in human T-leukaemia cell line (Jurkat)”, in “Proceedings of the International Conference on Electromagnetic Fields, Health and Environment”, Madeira Island (Portugal), 27-29 April 2006.

 

International Conference

P. Lamberti, S. Romeo, A. Sannino, M.R. Scarfì, V. Tucci, O. Zeni, L. Zeni, A Numerical Study of Electroporation Dynamics in Mammalian Cells Under Multiple Nanosecond Electric Pulses, BioEM2013, June 10-15, Thessaloniki, Greece.

S. Romeo, G. Pataro, A. Sannino, O. Zeni, G. Ferrari, M. R. Scarfì, L. Zeni, High voltage, ns electric pulse exposure of liquid media for evaluation of metal release from electrodes, BioEM2013, June 10-15, Thessaloniki, Greece.

S. Romeo, A. Sannino, O. Zeni, R. Massa, M.R. Scarfì, P. Bifulco, M. Cesarelli, R. d’Angelo, Repetitive exposure to a static magnetic field: cytoxicity investigations on human fetal lung fibroblasts, BioEM2013, June 10-15, Thessaloniki, Greece

A. Sannino, O. Zeni, S. Romeo, R. Massa, G. Gialanella, G. Grossi, L. Manti, Vijayalaxmi, M.R. Scarfì, Induction of adaptive response in human lymphocytes pre-exposed to radiofrequency fields and challenged with ionizing radiation, BioEM2013, June 10-15, Thessaloniki, Greece

S. Romeo, G. Pataro, A. Sannino, O. Zeni, G. Ferrari, M. R. Scarfì, Nanosecond, High-Voltage Pulse Generator for Electric Pulse Application to Low Conductivity Liquid Media, Bioelec trochemistry 2013, March 17-21, 2013, Bochum, Germany.

 S. Romeo, A. Sannino, O. Zeni, M. R. Scarfì, R. Massa, R. d’Angelo. Occupational exposure to statiuc magnetic fields associated to MRI: an INAIL-IREA project, International School of Bioelectromagnetics “Alessandro Chiabrera”, November 25 – December 1 2012, Erice (TP), Italy.

S. Romeo, O. Zeni, A. Sannino, M.R. Scarfì, L. Zeni, A modified Blumlein system with fiber optic-based switch control for high voltage, ns, pulse generation, 2012 International Conference Bio and Food Electrotechnologies, September 26-28, 2012, Salerno.

S. Romeo, L. Zeni, A. Sannino, M. R. Scarfì, P. T. Vernier, O. Zeni, Investigating the role of pulse repetition rate in modulating cellular response to high voltage, nanosecond electric pulses, 2012 IEEE International Power Modulator and High Voltage Conference, June 3-7, 2012, San Diego, CA.

A. Sannino, O. Zeni, R. Massa, M. Sarti, S. Romeo, Vijayalaxmi, M.R. Scarfì, “Adaptive response induced by UMTS signal in human blood lymphocytes”, BEMS 33nd Annual Meeting, Halifax, Canada, 12-17 June 2011.

 

O. Zeni, A. Sannino, M. Sarti, R. Massa, M.R. Scarfì, “Radiofrequency radiation at 1950 MHz UMTS signal does not induce cellular response in neuronal like PC12 cells”, 10th EBEA congress, Rome, Italy, 21-24 February 2011.

 

A. Sannino, O. Zeni, M. Sarti, Vijayalaxmi, S. Romeo, M.A. Belisario, M.R. Scarfì, “ Characterization of Radiofrequency Radiation-induced Adaptive Response in Human Peripheral Blood Lymphocytes”, BEMS 32nd Annual Meeting, Seoul, Korea, 14-18 June 2010.

 

O. Zeni, A. Sannino, M. Sarti, R. Massa, M.R. Scarfì, “Cytotoxicity investigation on neuronal-like PC12 cells exposed to 1950 MHz radiofrequency radiation”, BEMS 32nd Annual Meeting, Seoul, Korea, 14-18 June 2010.

 

O. Zeni, A. Sannino, M. Sarti, F. Brescia, R. Massa, S. Romeo, M.R. Scarfì,  “In Vitro Effects of Exposures And Co-Exposures to 1950 MHz Radiofrequency radiation: Ongoing Activities at CNR-IREA”, COST BM0704 workshop, Bordeaux, France,  27-28 May 2010.

 

A. Sannino, O. Zeni, M. Sarti, Vijayalaxmi, S. Romeo, M.R. Scarfì, “Radiofrequency radiation is capable of inducing Adaptive Response in human blood lymphocytes”,  European Conference on Antennas and Propagation 2010, Barcelona, Spain, 12-16 April 2010.

 

A. Sannino, M. Sarti, S.B. Reddy, T.J. Prihoda, Vijayalaxmi, M.R. Scarfì, “Induction of Adaptive Response in Human Lymphocytes Exposed to Radiofrequency Radiation”, Bioem2009, Davos, Switzerland, 14-19 June 2009.

 

F. Brescia, M. Sarti, R Massa, M.L. Calabrese, A. Sannino, M.R. Scarfì, “Reactive oxygen species formation is not enhanced by exposure to UMTS 1950 MHz radiation and co-exposure to ferrous ions in Jurkat cells”, Bioem2009, Davos, Switzerland, 14-19 June 2009.

 

A. Sannino, G. Di Costanzo, M. Sarti, O. Zeni , M.R. Scarfì, “ Evaluation of genotoxic effects in human fibroblasts following  900 MHz RF exposures and co-exposures to MX”, 8th EBEA congress, Bordeaux, France, 10-13 april 2007.

 

R. Palumbo, F. Brescia, A. Sannino, M. Sarti, M.R. Scarfì, “Exposure to 900MHz radiofrequency radiation induces caspase-3 activation in human T cells”, 8th EBEA congress, Bordeaux, France, 10-13 april 2007.

 

R. Palumbo, F. Brescia, D. Capasso, A. Sannino, M. Sarti, R. Pinto, F. Bersani, M.R. Scarfì, “900 MHz Radiofrequency Radiation induces caspase-3 activation in proliferating Human Peripheral Blood Lymphocytes”, 28th Annual Meeting of the Bioelectromagnetic Society, Cancun, Mexico, 11-15 June 2006.

 

A. Sannino, G. d’Ambrosio, R. Massa, M. Calabrese, G. Petraglia, P. Mita, M. Sarti, M.R. Scarfì, “Evaluation of cytotoxic and genotoxic effects in human peripheral blood leukocytes following exposure to 1950 MHz modulated signal”, International School of Bioelectromagnetics “Alessandro Chiabrera”, 2nd Course “Electromagnetic Fields and Genotoxicity” Erice (Sicily, Italy), 3-9 October 2005.

 

A. Sannino, P. Mita, G. d’Ambrosio, R. Massa, M. Calabrese, M. Sarti, M.R. Scarfì, “Evaluation of cytotoxic and genotoxic effects in human peripheral blood leukocytes following exposure to UMTS modulated signal”, BIOem 2005, Dublino, Irlanda,19-24 June 2005.

 

M. Calabrese, G. Castello, G. d’Ambrosio, F. Izzo, G.F. Grossi, R. Massa, M. Napolitano, G. Petraglia, A. Sannino, M. Sarti, P. Scampoli, M.R. Scarfì, O. Zeni, “A project in the framework of the “Campania EU-Region center of competence on information and communication technologies” related to the evaluation of cancer related endpoints in mammalian cells following in vitro exposures to UMTS radiofrequency signal”, 26th Annual Meeting of the Bioelectromagnetic Society, Washington DC, 21-24 June 2004.

 

M. Calabrese, G. Castello, G. d’Ambrosio, F. Izzo, G.F. Grossi, R. Massa, M. Napolitano, G. Petraglia, A. Sannino, M. Sarti, P. Scampoli, M.R. Scarfì, O. Zeni, ““WHITER”: Wireless technology health risks, a coordinated bioelectromagnetic research project”, ICNIRP- International NIR Workshop & Symposium, Seville, Spain, 20-22 May 2004.

 

M.R. Scarfì, P. Olla, A. Sannino, F. Bersani, A. Schiavoni, A. Antolini, O. Zeni,  “Absence of micronucleus induction in human peripheral blood lymphocytes following exposures to 900 MHz Radiofrequency radiation”, 24th Annual meeting of the Bioelectromagnetic Society,  Quebec City, Quebec, Canada, 23-27 June 2002.

 

National Conference

 

S. Romeo, A. Sannino, O. Zeni, M. R. Scarfì, R. Massa, R. d’Angelo. Valutazione dell’esposizione occupazionale ai campi magnetici statici in uso per la Risonanza Magnetica: un progetto INAIL-IREA, 19° Convegno di Igiene Industriale – Le Giornate di Corvara, April 3-5, 2013, Corvara (BZ)

 

A. Sannino, O. Zeni, S. Romeo, R. Massa, Vijayalaxmi, M.R. Scarfì: Radiofrequency-Induced Adaptive Response in Human Lymphocytes cultured in vitro. II° Convegno Nazionale "Interazioni fra Campi Elettromagnetici e Biosistemi ", Bologna, 27 – 29 Giugno, 2012.

 

A. Sannino, O. Zeni, S. Romeo, R. Massa, Vijayalaxmi, M.R. Scarfì. Risposta adattativa indotta da esposizioni a radiofrequenza in linfociti umani coltivati in vitro. CISAM, Pisa, 30-31 maggio 2012.

 

A. Sannino, O. Zeni, M. Sarti, Vijayalaxmi, S. Romeo, M.A. Belisario, M.R. Scarfì, “Adaptive response induced by radiofrequency radiation in human lymphocytes”, Convegno "Attività di Ricerca del Centro Interuniversitario ICEmB a Venti Anni dalla Sua Costituzione", Genova, 25-26 febbraio 2010.

 

M. Calabrese, G. Castello, G. d’Ambrosio, F. Izzo, G.F. Grossi, R. Massa, M. Napolitano, G. Petraglia, A. Sannino, M. Sarti, P. Scampoli, M.R. Scarfì, O. Zeni, “Wither: wireless technology health risks”, Un progetto coordinato sulla valutazione di eventuali effetti cancerogeni in cellule esposte a segnali UMTS, XV RINEM, Cagliari, 13-16 settembre 2004.

 

M.R. Scarfi', O. Zeni, M. Sarti, A. Perrotta, M. Romanò, A. Sannino, R. Di Pietro, M.B. Lioi, R. Barbieri, “Valutazione dell'induzione di effetti genotossici in colture cellulari di mammifero esposte a campi elettromagnetici di bassa e di alta frequenza”, III Convegno Nazionale del Progetto MIUR/CNR-ENEA “Salvaguardia dell’Uomo e dell’ambiente dalle emissioni elettromagnetiche”, Interazione tra campi elettromagnetici e soggetti esposti.  Roma, 1-2 aprile 2004.

A. Sannino, O. Zeni, S. Romeo, R. Massa, MR. Scarfi. "Adverse and beneficial effects in Chinese hamster lung fibroblast cells following radiofrequency exposure". Bioelectromagnetics. 2017. doi: 10.1002/bem.22034. [Epub ahead of print]

 

S. Romeo, A. Sannino, M.R. Scarfì, R. Massa, R. d’Angelo, O. Zeni, “Lack of effects on key cellular parameters of MRC-5 human lung fibroblasts exposed to 370 mT static magnetic field”, Sci Rep. 2016 14;6:19398. doi: 10.1038/srep19398.

  

P. Lamberti, S. Romeo, A. Sannino, L. Zeni, O. Zeni, “The Role of Pulse Repetition Rate in nsPEF-induced Electroporation: A Biological and Numerical Investigation”. IEEE Trans Biomed Eng. 2015. [Epub ahead of print]

Presentations

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